Procesos y Tecnologías en Alimentos

Deshidratación
Simulación y modelamiento de procesos
Ingeniería de procesos alimentarios
Preservación de alimentos por nuevas tecnologías
Compuestos bioactivos, propiedades biológicas y funcionales
Química analítica, Calidad e inocuidad de los alimentos

Investigadores

Dr. Antonio Vega Gálvez

avegag@userena.cl

+51 2 204305

1. Vega-Gálvez, A., Miranda, M., Vergara, J., Uribe, E., Puente, L., Martínez, E. (2010). Nutrition facts and functional potential of quinoa (Chenopodium quinoa willd.), an ancient andean grain: a Review. Journal of the Science of Food and Agriculture. 90(15), 2541-2547. https://doi.org/10.1002/jsfa.4158

2. Lemus-Mondaca, R., Vega-Gálvez, A., Zura-Bravo, L., Ah-Hen, K. (2012). Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chemistry. 132, 1121-1132. https://doi.org/10.1016/j.foodchem.2011.11.140.

3. Vega-Gálvez, A., Di Scala, K., Rodríguez, K., Lemus-Mondaca, R., Miranda, M., López, J., Perez-Won, M. (2009). Effect of air-drying temperature on physico-chemical properties, antioxidant capacity, colour and total phenolic content of red pepper (Capsicum annuum, L. var. Hungarian). Food Chemistry. 117, 647-653 1. https://doi.org/10.1016/j.foodchem.2009.04.066.

4. Vega-Gálvez, A., Ah-Hen, K., Chacana, M., Martínez-Monzó, J., García-Segovia, P., Roberto Lemus-Mondaca, Karina Di Scala. (2012). Effect of temperature and air velocity on drying kinetics, antioxidant capacity, total phenolic content, colour, texture and microstructure of apple (var. Granny Smith) slices. Food Chemistry. 132. 51–59. https://doi.org/10.1016/j.foodchem.2011.10.029.

5. Miranda, M., Maureira, H., Rodríguez, K., Vega-Gálvez, A. (2009). Influence of temperature on the drying kinetics, physicochemical properties, and antioxidant capacity of aloe vera (Aloe barbadensis miller) gel. Journal of Food Engineering. 91(1), 297-304. https://doi.org/10.1016/j.jfoodeng.2008.09.007.

6. Vega-Gálvez, A., Lemus-Mondaca, R., Bilbao-Sáinz, C., Fito, P., Andrés, A. (2008). Effect of air drying temperature on the quality of rehydrated dried red bell pepper (var. Lamuyo). Journal of Food Engineering. 85, 42-50. https://doi.org/10.1016/j.jfoodeng.2007.06.032.

7. Miranda, M., Vega-Gálvez, A., López, J., Parada, G., Sanders, M., Aranda, M., Uribe, E., Di Scala, K. (2010). Impact of air-drying temperature on nutritional properties, total phenolics content and antioxidant capacity of quinoa seeds (Chenopodium quinoa Willd.). Industrial Crop and Products-An International Journal. 32(3), 258-263. https://doi.org/10.1016/j.indcrop.2010.04.019.

8. López, J., Uribe, E., Vega-Gálvez, A., Miranda, M., Vergara, J., Gonzalez, E., Di Scala, K. (2010). Effect of air temperature on drying kinetics, vitamin C, antioxidant activity, total phenolic content, non-enzymatic browning and firmness of blueberries variety O´Neil. Food and Bioprocess Technology, 3, 772–777. https://doi.org/10.1007/s11947-009-0306-8

9. Vega, A., Uribe, E., Lemus, R. Miranda, M. (2007). Hot-air drying characteristics of aloe vera (Aloe barbadensis Miller) and influence of temperature on kinetics parameters. LWT - Food Science and Technology. 40(10), 1698-1707. https://doi.org/10.1016/j.lwt.2007.01.001.

10. Nuñez-Mancilla, Y., Vega-Gálvez, A., Pérez-Won, M., Zura, L., Garcia-Segovia, P., Di Scala K. (2014). Effect of osmotic dehydration under high hydrostatic pressure on microstructure, functional properties and bioactive compounds of strawberry (Fragaria vesca). Food and Bioprocess Technology: An International Journal. 7(2), 516-524. https://doi.org/10.1007/s11947-013-1052-5.

11. Puente-Díaz, L., Ah-Hen, K., Vega-Gálvez, A., Lemus-Mondaca, R., Di Scala, K. (2013). Combined infrared-convective drying of murta (Ugni molinae Turcz) berries: Kinetic modelling and quality assessment. Drying Technology. 31(3), 329-338. https://doi.org/10.1080/07373937.2012.736113.

12. Vega-Gálvez, A., Miranda, M., Clavería, R., Quispe, I., Vergara, J., Uribe, E., Paez, H., Di Scala, K. (2011). Effect of air temperature on drying kinetics and quality characteristics of osmo-treated jumbo squid (Dosidicus gigas). LWT - Food Science and Technology. 44, 16-23. https://doi.org/10.1016/j.lwt.2010.06.012.

13. Vega-Gálvez, A., Uribe, E., Perez,M, Tabilo-Munizaga G., Vergara, J., Garcia-Segovia, P., Lara, E., Di Scala, K. (2011). Effect of high hydrostatic pressure pretreatment on drying kinetics, antioxidant activity, firmness and microstructure of Aloe Vera (Aloe Barbadensis Miller) gel. LWT - Food Science and Technology. 44 (2), 384-391. https://doi.org/10.1016/j.foodchem.2011.05.074.

14. Tello-Ireland, C., Lemus-Mondaca, R., Vega-Gálvez, A., López, J., Di Scala, K. (2011). Influence of hot-air temperature on drying kinetics, functional properties, colour, phycobiliproteins, antioxidant capacity, texture, and agar yield of alga Gracilaria chilensis. LWT - Food Science and Technology. 44(10), 2112-2118. https://doi.org/10.1016/j.lwt.2011.06.008.
15. Miranda, M., Vega-Gálvez, A., García, P., Shi, J., Di Scala, K., Xue, S., Uribe, E. (2010). Effect of temperature on structural properties of Aloe vera (Aloe barbadensis Miller) gel and Weibull distribution for modelling drying process. Food and Bioproducts Processing. 88, 138-144. https://doi.org/10.1016/j.fbp.2009.06.001.

Dra. Vilbett Briones Labarca

vbriones@userena.cl

+512 204327

Dra. Issis Quispe

Iquispe@userena.cl

+51-2334661 / 2334675

Msc Elsa Uribe

muribe@userena.cl

+51 2334808

1. Elsa Uribe, Antonio Vega-Gálvez, Vivian García, Alexis Pastén, Katia Rodríguez, Jéssica López & Karina Di Scala (2020). Evaluation of physicochemical composition and bioactivity of a red seaweed (Pyropia orbicularis) as affected by different drying technologies. Drying Technology, vol. 38, Nº 9, 1218–1230. https://doi.org/10.1080/07373937.2019.1628771

2. Jonathan Cisternas-Jamet; Ricardo Salvatierra-Martínez; Antonio Vega-Gálvez; Alexandra Stoll; Elsa Uribe; María Gabriela Goñi (2020). Biochemical composition as a function of fruit maturity stage of bell pepper (Capsicum annum) inoculated with Bacillus amyloliquefaciens. Scientia Horticulturae Volume 263, 15 March 2020, 109107 https://doi.org/10.1016/j.scienta.2019.109107

3. E. Uribe, C. M. Pardo-Orellana, A. Vega-Gálvez, K. S. Ah-Hen, A. Pastén, V. García & S. P. Aubourg (2019). Effect of drying methods on bioactive compounds, nutritional, antioxidant, and antidiabetic potential of brown alga Durvillaea antarctica. Drying Technology; https://doi.org/10.1080/07373937.2019.1679830.

4. Jonathan Cisternas-Jamet, Ricardo Salvatierra-Martínez, Antonio Vega-Gálvez, Elsa Uribe, María G Goñi and Alexandra Stoll (2019). Root inoculation of green bell pepper (Capsic annum) with Bacillus amyloliquefaciens BBC047: effect on biochemical composition and antioxidant capacity. Journal of the Science of Food and Agriculture, vol. 99; 5131-5139. DOI 10.1002/jsfa.9758.

5. Uribe, E., Vega-Gálvez, A., García, V., Pasten, A., López, J., & Goñi, G. (2019). Effect of different drying methods on phytochemical content, amino acid and fatty acid profiles of green seaweed, Ulva spp. Journal of Applied Phycology, 31: 1967-1979. https://doi.org/10.1007/s10811-018-1686-9.

6. Antonio Vega-Gálvez, Issis Quispe-Fuentes, Elsa Uribe, Javier Martinez-Monzo, Alexis Pasten & Roberto Lemus-Mondaca (2019). Bioactive compounds and physicochemical characterization of dried apricot (Prunus armeniaca L.) as affected by different drying temperaturas. VOL. 17, NO. 1, 297–306 https://doi.org/10.1080/19476337.2019.1577918.

7. Elsa Uribe, Antonio Vega-Gálvez, Valentina Heredia, Alexis Pastén, Karina Di Scala (2018). An edible red seaweed (Pyropia orbicularis): influence of vacuum-drying on physicochemical composition, bioactive compounds, antioxidant capacity and pigments. Journal of Applied Phycology, 30: 673-683. https://doi.org/10.1007/s10811-017-1240-1.

8. Jéssica López, Antonio Vega-Gálvez, Cristina Bilbao-Sainz, Bor-Sen Chiou, Elsa Uribe, Issis Quispe-Fuentes (2017). Influence of vacuum drying temperature on: Physico-chemical composition and antioxidant properties of murta berries. Journal of Food Process Engineering. e12569; 1-9; https://doi.org/10.1111/jfpe.12569.

9. Margarita Miranda, Marcos Trigo, Elsa Uribe, Antonio Vega-Gálvez and Santiago P. Aubourg (2017). Enhancement of the rancidity stability in a marine-oil model by addition of saponin-free quinoa (Chenopodium quinoa Willd.) ethanol extracts. European Journal of Lipid Science and Technology 119, 1600291. https://doi.org/10.1002/ejlt.201600291.

10. Alexis Pasten, Elsa Uribe, Karina Stucken, Angela Rodrìguez, Antonio Vega-Gálvez (2017). Drying of olive waste cake (cv. Arbequina) for recovery of high-value products. Waste and Biomass Valorization. https://doi.org/10.1007/s12649-017-0187-4.

11. Elsa Uribe, Antonio Vega-Gálvez, Valeria Vásquez, Roberto Lemus-Mondaca, Loreto Callejas, Alexis Pastén (2017). Hot-air drying characteristics and energetic requirement of the edible brown seaweed Durvillaea Antarctica. Journal of Food Processing and Preservation. https://doi.org/10.1111/jfpp.13313.

12. López, Jéssica; Ah- Hen, Kong Shun; Vega-Gálvez, Antonio; Morales-Tapia, Amelia; García-Segovia, Purificación; Uribe, Elsa (2016). Effects of drying methods on quality attributes of murta (Ugni molinae Turcz) berries: bioactivity, nutritional aspects, texture profile, microstructure and functional properties. Journal of Food Process Engineering. https://doi.org/10.1111/jfpe.12511.

13. Elsa Uribe, Daniela Marín, Antonio Vega-Gálvez, Issis Quispe-Fuentes, Angela Rodríguez (2016). Assessment of vacuum-dried Mentha piperita L. as a source of natural antioxidants. Food Chemistry 190; 559-565. https://doi.org/10.1016/j.foodchem.2015.05.108.

14. Elsa Uribe, Alvaro Delgadillo, Issis Quispe-Fuentes, Claudia Giovagnoli-Vicuña and Liliana Zura-Bravo (2015). Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit. Journal of Chemistry Volume 2015, Article ID 347532, 8 pages http://dx.doi.org/10.1155/2015/347532.

15. Rosario Goyeneche, Sara Roura, Alejandra Ponce, Antonio Vega-Gálvez, Issis Quispe-Fuentes, Elsa Uribe, Karina Di Scala (2015). Chemical characterization and antioxidant capacity of red radish (Raphanus sativus L.) leaves and roots. Journal of Functional Foods 16; 256-264. https://doi.org/10.1016/j.jff.2015.04.049

Msc Alexis Pastén Contreras

afpasten@userena.cl

Laboratorios asociados

Laboratorio de Bioquímica
Laboratorio de Análisis Químico
Laboratorio de Evaluación Sensorial
Laboratorio de investigación 1
Laboratorio de investigación 2
Laboratorio de investigación 3
Laboratorio de Secado

Proyectos asociados

Vilbett Briones FONDEF ID19I10313 Desarrollo de bebidas proteicas en base a quinoa, frutas y hortalizas, enriquecidas en compuestos bioactivos, con mejorada digestibilidad y atributos sensoriales, utilizando tecnologías no térmicas.

Bíoprocesos

Tecnología de enzimas para bioprocesos
Ingeniería en proteínas y Evolución dirigida de enzimas para el mejoramiento de enzimas para condiciones no naturales
Valorización de subproductos alimentarios
Ingeniería de inmovilización de enzimas
Aplicación y adaptación de enzimas a procesos alimentarios
Ecología microbiana de alimentos

Investigadores

Dr. Karina Stucken

kstucken@userena.cl

+51 2204397

1. Vega-Gálvez, Rodriguez A, Stucken K (2020). Antioxidant, functional properties and health‐promoting potential of native South American berries: A review. Journal of the Science of Food and Agriculture https://doi.org/10.1002/jsfa.10621

2. Springstein BL, Arévalo S, Helbig AO, Herrero A, Stucken K, Flores E, Dagan T (2020) A novel septal protein of multicellular heterocystous cyanobacteria is associated with the divisome. Molecular Microbiology 2020; 00: 1– 15. https://doi.org/10.1111/mmi.14483

3. Springstein BL, Woehle C, Weissenbach J, Helbig AO, Dagan T, Stucken K. Identification and characterization of novel filament-forming proteins in cyanobacteria. Scientific Reports 10, 1894 (2020). https://doi.org/10.1038/s41598-020-58726-9

4. Zura-Bravo L, Rodriguez A, Stucken K, Castillo L, Ah-Hen KS, García-Segovia P, Vega-Gálvez A (2019). Nutritional and organoleptic properties of murta (Ugni molinae Turcz) berries impregnated with Lactobacillus casei var. rhamnosus and dehydrated by different methods. Food Chemistry 299:125117. https://doi.org/10.1016/j.foodchem.2019.125117

5. Zura-Bravo L, Rodriguez A, Stucken K, Vega-Gálvez A (2019). Drying kinetics of probiotic-impregnated murta (Ugni molinae T.) berries. Journal of Food Science and Technology. 56(1):103-113. https://doi.org/10.1007/s13197-018-3463-9

6. Torres-Ossandón MJ, Vega-Gálvez A, López J, Stucken K, Romero J, Di Scala, K (2018). Effects of high hydrostatic pressure processing and supercritical fluid extraction on bioactive compounds and antioxidant capacity of Cape gooseberry pulp (Physalis peruviana L.) The Journal of Supercritical Fluids 138:215-220. https://doi.org/10.1016/j.supflu.2018.05.005

7. Roco T, Torres-Ossandon MJ, Briones-Labarca V, Reyes JE, Tabilo-Munizaga G, Stucken K, Lemus-Mondaca R, Pérez-Won, M (2018). Effect of high hydrostatic pressure treatment on physical parameters, ultrastructure and shelf life of pre- and post-rigor mortis palm ruff (Seriolella violacea) under chilled storage. Food Research International 108:192-212. https://doi.org/10.1016/j.foodres.2018.03.009

8. Koch R, Kupczok A, Stucken K, Ilhan J, Hammerschmidt K, Dagan T (2017). Plasticity first: molecular signatures of a complex morphological trait in filamentous cyanobacteria. BMC Evol Biol 17:209. https://doi.org/10.1186/s12862-017-1053-5

9. Weissenbach J, Ilhan J, Bogumil D, Hülter N, Stucken K, Dagan T (2017). Evolution of chaperonin gene duplication in Stigonematalean cyanobacteria (subsection V). Genome Biol Evol 9:241-252. https://doi.org/10.1093/gbe/evw287.

10. Stucken K, John U, Cembella A, Soto-Liebe K, Vásquez M (2014). Impact of nitrogen sources on gene expression and toxin production in the diazotroph Cylindrospermopsis raciborskii CS-505 and non-diazotroph Raphidiopsis brookii D9. Toxins (Basel) 6:1896-915. https://doi.org/10.3390/toxins6061896

11. Stucken K, Koch R, Dagan T (2013). Cyanobacterial defense mechanisms against foreign DNA transfer and their impact on genetic engineering. Biol Res, 46:373–382. http://dx.doi.org/10.4067/S0716-97602013000400009

12. Dagan T, Roettger M, Stucken K, Landan G, Koch R, Major P, Gould SB, Goremykin VV, Rippka R, Tandeau de Marsac N, Gugger M, Lockhart PJ, Allen JF, Brune I, Maus I, Pühler A, Martin WF (2013). Genomes of Stigonematalean cyanobacteria (subsection V) and the evolution of oxygenic photosynthesis from prokaryotes to plastids. Genome Biol Evol 5:31-44. https://dx.doi.org/10.1093%2Fgbe%2Fevs117

13. Stucken K, Ilhan J, Röttger M, Dagan T, Martin W (2012). Transformation and conjugal transfer of foreign genes into the filamentous multicellular cyanobacteria (Subsection V) Fischerella and Chlorogloeopsis. Curr Microbiol 65:552-60. https://doi.org/10.1007/s00284-012-0193-5

14. Stucken K, John U, Cembella A, Murillo AA, Soto-Liebe K, Fuentes JJ, Friedel M, Plominsky A, Vásquez M, Glöckner G (2010). Comparison of the smallest known genomes of multicellular and toxic cyanobacteria: minimal gene sets for linked traits and the evolutionary implications. PLoS ONE 5: e9235. https://doi.org/10.1371/journal.pone.0009235

15. Soto-Liebe K, Murillo AA, Krock B, Stucken K, Fuentes-Valdés JJ, Trefault N, Cembella A, Vásquez M (2010). Reassessment of the toxin profile of Cylindrospermopsis raciborskii T3 and function of putative sulfotransferases in synthesis of sulfated and sulfonated PSP toxins. Toxicon, 56:1350-61. https://doi.org/10.1016/j.toxicon.2010.07.022

Dr. Ronny Martínez

remartinez@userena.cl

+51 2334661

1. Belsare, K.D.; Ruff, A. J.; Martinez, R.; Schwaneberg, U. (2020) Insights on intermolecular FMN-heme domain interaction and the role of linker length in cytochrome P450cin fusion proteins. Biological Chemistry Ahead of Print https://doi.org/10.1515/hsz-2020-0134

2. Rodríguez, K.; Martinez, R.; Bernal, C. (2020) Selective immobilization of Bacillus subtilis lipase A from cell culture supernatant: Improving catalytic performance and thermal resistance. Process Biochemistry. Volume 92, 214-223. https://doi.org/10.1016/j.procbio.2020.01.013

3. Martínez, R; Bernal, C; Álvarez, R; Concha, C; Araya, F; Cabrera, R; Dhoke, G. V.; Davari, M. D. (2020) Deletion and Randomization of Structurally Variable Regions in B. subtilis Lipase A (BSLA) Alter Its Stability and Hydrolytic Performance Against Long Chain Fatty Acid Esters. International Journal of Molecular Sciences 21 (6), 1990 https://doi.org/10.3390/ijms21061990

4. Novoa, C., Dhoke, G. V., Mate, D. M., Martínez, R., Haarmann, T., Schreiter, M., Schwaneberg, U. (2019). KnowVolution of a fungal laccase toward alkaline pH. ChemBioChem. https://doi:10.1002/cbic.201800807

5. V Vásquez, R Martínez, C Bernal (2019) Enzyme-assisted extraction of proteins from the seaweeds Macrocystis pyrifera and Chondracanthus chamissoi: characterization of the extracts and their bioactive potential. Journal of Applied Phycology, 31 (3), 1999-2010 https://doi.org/10.1007/s10811-018-1712-y

6. Körfer, G., Novoa, C., Kern, J., Balla, E., Grütering, C., Davari MD., Martinez, R, Vojcic, L., Schwaneberg, U., (2018) Directed evolution of an acid Yersinia mollaretii phytase for broadened activity at neutral pH. Applied Microbiology and Biotechnology. 102(22) 9607-9602. https://doi.org/10.1007/s00253-018-9308-7

7. Bernal, C., Rodríguez, K., Martínez, R. (2018) Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts. Biotechnology Advances, 36(5) pp 1470-1480. https://doi.org/10.1016/j.biotechadv.2018.06.002

8. Islam, S., Mate, D., Martínez R., Jakob, F., and Schwaneberg, U. (2018) A robust protocol for directed aryl sulfotransferase evolution toward the carbohydrate building block GlcNAc. Biotechnology and Bioengineering. DOI: 10.1002/bit.26535. https://doi.org/10.1002/bit.26535

9. Belsare, K., Horn, T., Ruff, AJ., Martinez, R., Magnusson, A., Holtmann, D., Schrader, J., Schwaneberg, U (2017) Directed evolution of P450cin for mediated electron transfer PEDS, 30(2), 119–127. https://doi.org/10.1093/protein/gzw072

10. Schatte, M., Bocola, M., Roth, T., Martinez, R., Kopetzki, E., Schwaneberg, U., & Bönitz-Dulat, M. (2016). Reporter Immobilization Assay (REIA) for Bioconjugating Reactions. Bioconjugate Chemistry. 27 (6), pp 1484–1492. https://doi.org/10.1021/acs.bioconjchem.6b00111

11. Körfer, G., Pitzler, C., Vojcic, L., Martinez, R., and Schwaneberg, U. (2016). In vitro flow cytometry-based screening platform for cellulase engineering. Scientific Reports, 6, 26128. https://doi.org/10.1038/srep26128

12. Vojcic, L., Pitzler, C., Körfer, G., Jakob, F., Martinez, R., Maurer, K.-H., & Schwaneberg, U. (2015). Advances in protease engineering for laundry detergents. New Biotechnology, 32, 629-634. https://doi.org/10.1016/j.nbt.2014.12.010

13. Lulsdorf, N., Vojcic, L., Hellmuth, H., Weber, T T., Mußmann, N., Martinez, R., Schwaneberg, U. (2015) A first continuous 4-aminoantipyrine (4-AAP)-based screening system for directed esterase evolution. Applied Microbiology and Biotechnology, 99(12), 5237-5246. https://doi.org/10.1007/s00253-015-6612-3

14. Lülsdorf, N., Pitzler, C., Biggel, M., Martinez, R., Vojcic, L., & Schwaneberg, U. (2015). A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels. Chem. Commun., 51(41) 8679–8682. https://doi.org/10.1039/C5CC01791B

15. Pitzler, C., Wirtz, G., Vojcic, L., Hiltl S., Böker, A., Martinez, R., Schwaneberg, U. (2014) A Fluorescent Hydrogel-Based Flow Cytometry High-Throughput Screening Platform for Hydrolytic Enzymes. Chemistry & Biology 21 (12), 1733-1742. https://doi.org/10.1016/j.chembiol.2014.10.018

Dra. Claudia Bernal

cbernal@userena.cl

+51 2334661

1. Vallejo-Castillo, V., Rodríguez-Stouvenel, A., Martínez, R., Bernal, C. Development of alginate-pectin microcapsules by the extrusion for encapsulation and controlled release of polyphenols from papaya (Carica papaya L.). Journal of Food Biochemistry. https://doi.org/10.1111/jfbc.13331. 2020

2. Origone, A., Barberis, S., Illanes, A., Guzmán, F., Camí, G., Liggieri, C., Martínez, R., Bernal, C. Improvement of enzymatic performance of Asclepias curassavica L. proteases by immobilization. Application to the synthesis of an antihypertensive peptide. Process Biochemistry. 2020. 95, 36-46 https://doi.org/10.1016/j.procbio.2020.05.013

3. K. Rodriguez, R. Martinez, C. Bernal. Selective immobilization of Bacillus subtilis lipase A from cell culture supernatant: Improving catalytic performance and thermal resistance. Process Biochemistry. 2020. 92, 214-223 https://doi.org/10.1016/j.procbio.2020.01.013

4. C. Tufiño, C. Bernal, C. Ottone, O. Romero, A. Illanes, L. Wilson. Synthesis with Immobilized Lipases and Downstream Processing of Ascorbyl Palmitate. Molecules 2019, 24, 3227. https://doi.org/10.3390/molecules24183227

5. V. Vásquez, R. Martinez, C. Bernal. Enzyme-assisted extraction of proteins from the seaweeds Macrocystis pyrifera and Chondracanthus chamissoi: characterization of the extracts and their bioactive potential. Journal of Applied Phycology. Journal of Applied Phycology. 2019. 31(3), pp. 1999-2010https://doi.org/10.1007/s10811-018-1712-y

6. C. Bernal, K. Rodriguez, R. Martinez. Integrating enzyme immobilization and protein engineering: an alternative path for the development of novel and improved industrial biocatalysts. Biotechnology Advances. 36, 2018, 1470–1480 https://doi.org/10.1016/j.biotechadv.2018.06.002

7. P. Urrutia, C. Bernal, L. Wilson, A. Illanes. Use of chitosan heterofunctionality for enzyme immobilization: β-galactosidase immobilization for galacto-oligosaccharide synthesis. International Journal of Biological Macromolecules. 116, 2018, 182-193 https://doi.org/10.1016/j.ijbiomac.2018.04.112

8. C. Bernal, J.C. Poveda-Jaramillo, M. Mesa. Raising the enzymatic performance of lipase and protease in the synthesis of sugar fatty acid esters, by combined ionic exchange -hydrophobic immobilization process on aminopropyl silica support. Chemical Engineering Journal 334 (2018) 760–767 https://doi.org/10.1016/j.cej.2017.10.082

9. Bernal C., Guzman F., Illanes A., Wilson L. Selective and eco-friendly synthesis of lipoaminoacid-based surfactants for food, using immobilized lipase and protease biocatalysts. Food Chemistry. 2018, 239, 189-195 https://doi.org/10.1016/j.foodchem.2017.06.105

10. Escobar S., Velasco-Lozano S., Lu C-H., Lin Y-F., Mesa M., Bernal C. López-Gallego F. Understanding the functional properties of bio-inorganic nanoflowers as biocatalysts by deciphering the metal-binding sites of enzymes. Journal of Materials Chemistry B. 2017 5, 4478-4486 https://doi.org/10.1039/C6TB03295H

11. Escobar S., Bernal C., Mesa M. Relationship between sol–gel conditions and enzyme stability: a case study with β-galactosidase/silica biocatalyst for whey hydrolysis. Journal of Biomaterials Science, Polymer Edition. 2015. 26 (16), 1126-1138 https://doi.org/10.1080/09205063.2015.1078929

12. Bernal Claudia, Illanes Andres, Wilson Lorena. Improvement of Efficiency in the Enzymatic Synthesis of Lactulose Palmitate. Journal of Agricultural and Food Chemistry. 2015, 63 (14), 3716-3724 https://doi.org/10.1021/jf505222x

13. Bernal Claudia, Illanes Andres, Wilson Lorena. Heterofunctional hydrophilic-hydrophobic porous silica as support for multipoint covalent immobilization of lipases: Application to lactulose palmitate synthesis. Langmuir 2014. 30(12), pp. 3557-3566 https://doi.org/10.1021/la4047512

14. Bernal C., Marciello M., Mesa M., Sierra L., Fernandez-Lorente G., Mateo C., Guisan J.M. Immobilisation and stabilisation of β-galactosidase from Kluyveromyces lactis using a glyoxyl support. International Dairy Journal 2013. 28(2), pp. 76-82. https://doi.org/10.1016/j.idairyj.2012.08.009

15. Bernal, C., Sierra, L., Mesa, M. Application of hierarchical porous silica with a stable large porosity for β-galactosidase immobilization. ChemCatChem 2011. 3(12), pp. 1948-1954. https://doi.org/10.1002/cctc.201100174

Laboratorios asociados

Laboratorio de Microbiología de Alimentos
Laboratorio de Tecnología Enzimática para Bioprocesos (TEB)

Proyectos asociados

Karina Stucken, FONDECYT Regular código 11170842‬ Cyanobacteria as soil regenerating agents: Characterization of biological crust cyanobacteria from Coquimbo drylands for bioprospecting and rehabilitating degraded soils.
Claudia Bernal FONDECYT Regular código 1190260 Galactaric acid production from pectin: Integration of multienzymatic systems through enzyme co-immobilization on glyoxyl silica.
Ronny Martínez FIC-R 30485942-0 Calamar Gigante: Desarrollo de Bioproductos Marinos
Ronny Martínez FONDECYT regular código 1200466 Improving enzymatic detection of histamine through protein engineering for food quality applications

Líneas de Investigación

Procesos y Tecnologías en Alimentos

Investigadores

Laboratorios asociados

Proyectos asociados

Bíoprocesos

Investigadores

Laboratorios asociados

Proyectos asociados